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首页> 外文OA文献 >Expression of a single-chain HLA class I molecule in a human cell line: presentation of exogenous peptide and processed antigen to cytotoxic T lymphocytes.
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Expression of a single-chain HLA class I molecule in a human cell line: presentation of exogenous peptide and processed antigen to cytotoxic T lymphocytes.

机译：单链HLA I类分子在人细胞系中的表达：向细胞毒性T淋巴细胞呈递外源肽和加工过的抗原。

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摘要

We have synthesized a recombinant gene encoding a single-chain HLA-A2/beta 2-microglobulin (beta 2m) molecule by linking beta 2m through its carboxyl terminus via a short peptide spacer to HLA-A2 (A*0201). This gene has been expressed in the beta 2m-deficient colorectal tumor cell line DLD-1. Transfection of this cell with the single-chain construct was associated with conformationally correct cell surface expression of a class I molecule of appropriate molecular mass. The single-chain HLA class I molecule presented either exogenously added peptide or (after interferon-gamma treatment) endogenously processed antigen to an influenza A matrix-specific, HLA-A2-restricted cytotoxic T-lymphocyte line. The need for interferon gamma for the processing and presentation of endogenous antigen suggests that DLD-1 has an antigen-processing defect that can be up-regulated, a feature that may be found in other carcinomas. Our data indicate that single-chain HLA class I constructs can form functional class I molecules capable of presenting endogenously processed antigens. Such molecules should be of use for functional studies, as well as providing potential anticancer immunotherapeutic agents or vaccines.
机译：我们已经合成了一个编码单链HLA-A2 /β2微球蛋白（β2m）分子的重组基因，该基因通过将β2m通过其羧基末端通过短肽间隔子连接到HLA-A2（A * 0201）。该基因已在beta 2m缺乏的结直肠肿瘤细胞系DLD-1中表达。用单链构建体转染该细胞与适当分子量的I类分子的构象正确的细胞表面表达有关。单链HLA I类分子将外源添加的肽或（经干扰素-γ处理后）内源性加工的抗原呈递给甲型流感病毒基质特异性HLA-A2限制的细胞毒性T淋巴细胞系。对于加工和呈递内源性抗原的干扰素γ的需求表明，DLD-1具有可上调的抗原加工缺陷，这一特征可能在其他癌症中发现。我们的数据表明，单链HLA I类构建体可以形成能够呈递内源性加工抗原的功能性I类分子。此类分子应用于功能研究，并提供潜在的抗癌免疫治疗剂或疫苗。

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